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Bleach and water solution A person might be able to eliminate tobacco stains on the fingers by: diluting 1 part bleach with 4 parts water soaking a nail brush in the solution and applying it to the stained areas leaving the solution on for a few minutes, then rinsing the fingers thoroughly They should then apply a moisturizing hand cream to prevent dry skin

Upon reaching 90% confluency, cells were rinsed with Dulbeccos phosphate-buffered saline (DPBS) without calcium and magnesium then detached using 1.5 mL of 0.25% trypsin EDTA/DPBS with polyvinylpyrrolidone for 1.5 min at 37 C

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