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Atomic model building and refinement To build the atomic model for the mTORC1RHEBRAGRagulator4E-BP1 complex on the membrane, we first fit our previous models into the cryo-EM map as a rigid body using UCSF ChimeraX 56 , with substituted models of mTOR and RAPTOR from AlphaFold2 prediction 57

Mucocele This is a condition wherein the inflamed gallbladder mucosa, in the absence of bacterial infection, continues to absorb water from bile and excretes mucus despite gallstone blockage of the bile ducts