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Colonies were used to inoculate 0.25 ml SC-ULH medium (1.47 g l 1 Synthetic Complete (Kaiser) Drop Out: Leu, His, Ura, 6.7 g l 1 yeast nitrogen base (without amino acids), 20 g l 1 d-(+)-glucose), pH set to 5.8 with hydrochloric acid) or SC-U medium (SC-ULH, 380 mg l 1 leucine, 76 mg l 1 histidine), respectively, in 96-deep-well plates equipped with a CR1296 sandwich cover (EnzyScreen)

In addition, DiR-labeled samples were prepared by adding 0.2 g DiR (the DiR dose in the pharmacokinetic experiment group was 5 g) to ethanol in the same methods

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66009-1-Ig, 1:5000), were purchased from Proteintech (Wuhan, Hubei, China)